Mitochondrial Membrane Potential Assay Kit (II) from Cell Signaling Technology

Product Specs
ItemMitochondrial Membrane Potential Assay Kit (II)
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number13296S
Quantity1 Kit
Research AreaScreening Technologies
TargetMitochondrial Membrane Potential Assay Kit
ApplicationsIF-IC, FC-FP
ReactivityAll

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Proteomic analysis of exosomes secreted during the epithelial-mesenchymal transition and potential biomarkers of mesenchymal high-grade serous ovarian carcinomaJournal of Ovarian ResearchNovember 29, 2023Germano Aguiar Ferreira, Carolina Hassibe Thomé, Clarice Izumi, Mariana Lopes Grassi, Guilherme Pauperio Lanfredi, Marcus Smolka, Vitor Marcel Faça, Francisco José Candido dos Reismitochondrial membrane potential was conducted using intracellular tetramethylrhodamine ethyl ester perchlorate (TMRE) (#13296, Cell Signaling), following the manufacturer’s instructions. To establish control, cell lines were incubated

(2) IRP1 mediated ferroptosis reverses temozolomide resistance in glioblastoma via affecting LCN2/FPN1 signaling axis depended on NFKB2iScienceAugust 18, 2023Yufei Lan, Tao Yang, Qu Yue, Zhao Wang, Xiangyang Zhong, Xin Luo, Boming Zuo, Manqing Zhang, Tianci Zeng, Boyang Liu, et al.using the JC-1 Assay Kit (Beyotime, C2006, China) and the TMRE Assay Kit (Cell Signaling Technology, #13296, MA, USA). According to the manufacturer’s protocols, Cells fixed on confocal dishes were stained with

(3) Pgam5-mediated PHB2 dephosphorylation contributes to endotoxemia-induced myocardial dysfunction by inhibiting mitophagy and the mitochondrial unfolded protein responseInternational journal of biological sciencesJanuary 1, 2023Cai C, Li Z, Zheng Z, Guo Z, Li Q, Deng S, Shi N, Ou Q, Zhou H, Guo Z, et al.health, was measured in this study 43. To do so, we used a Mitochondrial Membrane Potential Assay Kit (13296, Cell Signaling Technology, Danvers, MA, USA). This kit utilizes JC-1 staining, a fluorescent dye that

(4) PBDEs Found in House Dust Impact Human Lung Epithelial Cell HomeostasisToxicsFebruary 19, 2022Antonio Zandona, Karla Jagić, Marija Dvoršćak, Josip Madunić, Darija Klinčić, Maja Katalinićethyl ester perchlorate (TMRE) from the assay kit (Mitochondrial Membrane Potential Assay Kit (II), #13296, Cell Signaling Technology Europe, Leiden, The Netherlands). Namely, as described in our previous study

(5) Substrate Reduction Therapy Reverses Mitochondrial, mTOR, and Autophagy Alterations in a Cell Model of Gaucher DiseaseCellsSeptember 2, 2021Peng Y, Liou B, Lin Y, Fannin V, Zhang W, Feldman RA, Setchell KDR, Grabowski GA, Sun Y.medium was replaced with fresh neurobasal medium with 200 nM tetramethylrhodamine, ethyl ester (TMRE, 13296S, CST) added to each well and incubated at 37 °C for 20 min. For the control group, 50 µM carbonyl cyanide

(6) Aurora kinase A inhibition reverses the Warburg effect and elicits unique metabolic vulnerabilities in glioblastomaNature communicationsSeptember 1, 2021Nguyen TTT, Shang E, Shu C, Kim S, Mela A, Humala N, Mahajan A, Yang HW, Akman HO, Quinzii CM, et al.ethyl ester perchlorate) staining was performed according to the manufacturer’s instructions (CST, 13296S). Signal was detected on a LSRII flow cytometry (Becton–Dickinson, New Jersey) and the data were analyzed

(7) HIGD‑1B inhibits hypoxia‑induced mitochondrial fragmentation by regulating OPA1 cleavage in cardiomyocytesMolecular medicine reportsAugust 1, 2021Pang Y, Zhu Z, Wen Z, Lu J, Lin H, Tang M, Xu Z, Lu J.mitochondrial membrane potential was performed using a mitochondrial membrane potential assay kit (cat. no. 13296S; Cell Signaling Technologies, Inc.) according to the manufacturer's instructions. Flow cytometry for

(8) Quiescin sulfhydryl oxidase 1 promotes sorafenib-induced ferroptosis in hepatocellular carcinoma by driving EGFR endosomal trafficking and inhibiting NRF2 activationRedox biologyMay 1, 2021Sun J, Zhou C, Zhao Y, Zhang X, Chen W, Zhou Q, Hu B, Gao D, Raatz L, Wang Z, et al.potential (ΔΨm) were measured as previously described [43]. 1 × 106 cells were incubated with 200 nM TMRE (#13296, Cell signaling, USA) for 15 min at 37 °C. Cells were washed 3 times with PBS, harvested and suspended

(9) Epigenetic Targeting of Mcl-1 Is Synthetically Lethal with Bcl-xL/Bcl-2 Inhibition in Model Systems of GlioblastomaCancersAugust 1, 2020Shang E, Nguyen TTT, Shu C, Westhoff MA, Karpel-Massler G, Siegelin MD.USA), CST 4087S) to detect the subG1 fraction. TMRE staining (Mitochondrial Membrane Potential kit (CST 13296S)) was conducted to detect mitochondrial membrane potential. Collected data was analyzed by FlowJo software

(10) Sensing of ATP via the Purinergic Receptor P2RX7 Promotes CD8⁺ Trm Cell Generation by Enhancing Their Sensitivity to the Cytokine TGF-βImmunityJuly 14, 2020Borges da Silva H, Peng C, Wang H, Wanhainen KM, Ma C, Lopez S, Khoruts A, Zhang N, Jameson SC.M22426 Mitochondrial Membrane Potential Assay Kit (II) (TMRE),Cell Signaling Technologies,Cat no. 13296S Treg-Protector (anti-ARTC2 Nanobody) [S+16a],BioLegend,"Clone S+16a, Cat no. 149802; RRID:AB_2565494" ,, "Chemicals,

(11) Inhibition of HDAC1/2 Along with TRAP1 Causes Synthetic Lethality in Glioblastoma Model SystemsCellsJuly 10, 2020Nguyen TTT, Zhang Y, Shang E, Shu C, Quinzii CM, Westhoff MA, Karpel-Massler G, Siegelin MD.USA), CST 4087S). To detect mitochondrial membrane potential, Mitochondrial Membrane Potential kit (CST 13296S) was used. The FlowJo software (version 10.6.2; Tree Star, Ashland, OR, USA) was employed for the entire

(12) HDAC inhibitors elicit metabolic reprogramming by targeting super-enhancers in glioblastoma modelsThe Journal of clinical investigationJuly 1, 2020Nguyen TTT, Zhang Y, Shang E, Shu C, Torrini C, Zhao J, Bianchetti E, Mela A, Humala N, Mahajan A, et al.tetramethylrhodamine, ethyl ester (TMRE) (Mitochondrial Membrane Potential Kit, Cell Signaling Technology [CST], 13296S). The data were analyzed with FlowJo software, version 8.7.1 (Tree Star). In addition, mitochondria

(13) Integrated analysis of the molecular pathogenesis of FDXR-associated diseaseCell Death & DiseaseJune 4, 2020Jesse D. Slone et al.Vermont, United States), with excitation at 550 nm and emission at 580 nm (Cell Signaling Technology, #13296). Anterograde axonal transport assay in mouse retinal ganglion cells (RGCs) Seven-month-old B6;129

(14) TOM40 Inhibits Ovarian Cancer Cell Growth by Modulating Mitochondrial Function Including Intracellular ATP and ROS LevelsCancersMay 22, 2020Yang W, Shin HY, Cho H, Chung JY, Lee EJ, Kim JH, Kang ES.mitochondrial membrane potential assay was performed using the Membrane Potential Assay Kit (II) (#13296S, Cell Signaling Technology, Danvers, MA, USA) and MitoTracker Green FM (Thermo Fisher Scientific, Rockford,

(15) D609 protects retinal pigmented epithelium as a potential therapy for age-related macular degenerationSignal transduction and targeted therapyMarch 4, 2020Wang B, Wang L, Gu S, Yu Y, Huang H, Mo K, Xu H, Zeng F, Xiao Y, Peng L, et al.Mitochondrial membrane potential assay MMP was measured with a Mitochondrial Membrane Potential Assay Kit (CST, #13296S). Briefly, after culture in conditioned medium, cells were rinsed and washed 3 times with phosphate-buffered

(16) Activation of LXRβ inhibits tumor respiration and is synthetically lethal with Bcl-xL inhibitionEMBO molecular medicineOctober 1, 2019Nguyen TTT, Ishida CT, Shang E, Shu C, Torrini C, Zhang Y, Bianchetti E, Sanchez-Quintero MJ, Kleiner G, Quinzii CM, et al.following treatments of cells in strict accordance with the protocol provided by the manufacturer (#13296, CST, Inc), and loss of mitochondrial membrane potential was recorded by flow cytometry. For the flow

(17) Increased expression of heme-binding protein 1 early in Alzheimer's disease is linked to neurotoxicityeLifeAugust 27, 2019Yagensky O, Kohansal-Nodehi M, Gunaseelan S, Rabe T, Zafar S, Zerr I, Härtig W, Urlaub H, Chua JJ.Mitochondrial membrane potential was assayed with the Mitochondrial Membrane Potential Assay kit (Cell Signaling, 13296) according to the manufacturer’s guidelines. Briefly, potentiometric fluorescent TMRE dye (final concentration

(18) Knockdown of PRL-3 increases mitochondrial superoxide anion production through transcriptional regulation of RAP1Cancer Management and ResearchNovember 23, 2018Yongyong Yang, Shenyi Lian, Lin Meng, Zhihua Tian, Qin Feng, Yue Wang, Ping Wang, Like Qu, Chengchao Shousuperoxide anions, respectively. The cationic dye, tetramethylrhodamine ethyl ester perchlorate (TMRE) (#13296, Cell Signaling Technology) was used to detect the mitochondrial membrane potential in cells. HCT116 and

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Mitochondrial Membrane Potential Assay Kit (II) from Cell Signaling Technology

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Cell Signaling Technology

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Mitochondrial Membrane Potential Assay Kit (II) from Cell Signaling Technology

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